型号: Cdc42货号: 26905品牌: NewEast Biosciences参考价格: 4800元/个包装: 30微升交货期: 现货索取资料及报价
包装与价格： | 订货号 | 纯度规格 | 包装 | 价格(元) | | 26905 | | 30微升 | 4800 |
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| 型号: | Cdc42 | | 货号: | 26905 | | 品牌: | NewEast Biosciences | | 参考价格: | 4800 | | 交货期: | 现货 | | 产品别名: | Cdc42 GTP单抗抗体
Anti-Cdc42-GTP Monoclonal Antibody
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产品介绍 | 描述: Anti-Cdc42-GTP Monoclonal Antibody | | 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). | | 免疫原: Recombinant full length protein of active Cdc42 |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing | IP-蛋白质印迹:  IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008). | 免疫荧光: Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld’s hard set mounting medium (H-1500), and examined using a Zeiss inverted fluorescence microscope. |
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